Most of us have settled into a life without Pleasure Island, as depressing as that may be. You'll find a complete updated list of alternate clubs below however I thought I'd summarize that lengthy list with this one showing the best replacement clubs for those formerly located at PI.
If you liked 8TRAX, what is the best replacement? Antigua (Saturday nights)
If you liked Rock'n'Roll Beach Club, what is the best replacement? Margaritaville (Thursday, Friday, Saturday nights)
If you liked Motion, what is the best replacement? The Groove (Thursday, Friday, Saturday nights)
If you liked BET Soundstage, what is the best replacement? Any damn club in town is playing hip hop & rap! A shout-out to Bob Marley's for reggae!
And if you liked Mannequins, the only thing that comes close is DJ Dave Cannalte's Hospitality Industry Night at House of Blues! (Thursday nights) See: http://www.hob.com/venues/clubvenues/orlando/weekly/hospitality.asp